SKOV-3 Cell Migration
The Oris Pro 384 Cell Migration Assay was used to study motility of SKOV-3 ovarian cancer cells in response to the actin polymerization inhibitor, Cytochalasin D. Representative pre- and post-migration fluorescent images of the detection zone obtained using the Isocyte DL are shown for DiD-labeled cells migrating in the presence or absence of Cytochalasin D (Figure 2). The premigration area of the detection zone (i.e., the cell-free area) was calculated as 2 x 106 µm2 4 hours after cell seeding, with a nominal variance of ± 5% across all wells of the assay plate.
In the absence of Cytochalasin D, the SKOV-3 cells migrated into the detection zone and covered a 9 x 105 µm2 area, or about 45% area closure of the previously cell-free detection zone after a 16 hour incubation period. In contrast, cells treated with 0.06 µM Cytochalasin D only covered a 5.5 x 105 µm2 area of the detection zone, or about 28% area closure. The 4 µM concentration of Cytochalasin D completely prevented cell migration as indicated by essentially equivalent area closure in the pre- and post-migration wells (Figure 2A).