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May 15, 2014 (Vol. 34, No. 10)

Cell Culture Media for Fluorescence Imaging

Striking the Right Balance between Signal Strength and Long-Term Cell Health

  • A key challenge with live-cell fluorescence imaging is the ability to visualize weak fluorescent signals over background without inducing photobleaching or phototoxicity from high excitation light intensities. Most cell culture media contain components that emit significant autofluorescence when excited, thus negatively impacting fluorophore signal-to-noise (S:N) ratios. In turn, ...

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