Ademtech (www.ademtech. com) recently introduced the mRNAdembeads Purification kit for mRNA purification starting from total RNA. "The kit isolates the messengers in 20 minutes, and the RT-PCR can be directly performed in the presence of nanoparticles," according to Jerome Jolly, product manager. It is fully compatible with amplification enzymes activity.
"High-purity mRNA can be isolated from as little as 1 g of total RNA and concentrated in small volume less than 15 L," he says. Consequently, low expressed mRNA, such as the DFTR gene from pulmonary cells, can be purified, which is useful for sensitive microarrays.
"Soon," Jolly says, "Ademtech will present another version of this kit to directly purify mRNA from cell lysate and blood, reducing the amount of starting material to 100 cells." By year's end, the technology will be adapted for standard liquid-handling robots. Ademtech has also developed a new magnet, the Adem-Mag MSV, to facilitate purification of a large number of samples simultaneously.
Ademtech's superparamagnetic nanoparticles are available in sizes of 100, 200, 300, and 500 nm, all with a coefficient of variation of less than 20% and a magnetic oxide content greater than 70%. Surface area is up to 15 m2/g, for high sensitivity.
"This offers an easy, automatable, sensitive tool for immunoassays without sedimentation or aggregation associated with larger and polydispersed magnetic particles," Jolly says.
"The technology has been applied to produce different surface chemistriesCOOH or NH2or with biological activities brought by streptavidin, antibodies, or protein AG dedicated to proteomics and cell sorting."
At Bangs Laboratories (www. bangslabs.com), the new QuantumPlex M superparamagnetic multiplexing bead array is based on a polymer-based 6-micron magnetic particle dyed with the company's proprietary fluorophore, Starfire Red.
Designed for the flow cytometric analysis of a sample for multiple analytes or high throughput screening of multiple samples, the QuantumPlex M platform lets users see five distinct fluorescence peaks. Researchers can decorate particles with antibodies or DNA probes, for example, and excite those particles using 488- or 633-nm lasers.
Small labs, in particular, will benefit, according to Kathy Turner, technical services, because there is no additional equipment to buy. The particles work with any standard flow cytometer.
The BioMag ProMax product family recently added the Albumin Removal Kit and the Serum IgG Removal Kit. Albumin accounts for 5070% of the total protein in serum, and IgG accounts for 1020%. Removing these proteins facilitates detection of less abundant proteins in serum. The kits require no sample pretreatment, no columns, and no centrifugation.
Bangs also is continuing to expand its BioMag product line, Turner says. The particles are more than 90% iron oxide, irregularly shaped for a "tremendous" surface area, silanized to enhance functionality and bonding, and about 1.5 microns in diameter. Other products are in development but are, as yet, proprietary.
WaveSense (www.wavesense. com) focuses on xenographic retention chromatography, which fuses multiple dissimilar separations technologiesnotably magnetic separation, lateral flow separation immunoaffinity, and nucleoaffinity. "Both cellular and molecular assays can be performed with the simplicity of a test strip," according to Christopher Feistel, CEO. "Performance surpasses microtitre and flow cytometry formats."
The system, Feistel says, is akin to "a test strip on a rail." It creates flow resistance at the nano-scale and "works with the applied magnetic field to arrest magnetic particles coated with antibodies or oligo probes specific for the target cell or molecule," pulling the target into alignment for analysis, effectively concentrating the signal.
Only magnetic particles are captured in the flow path, he emphasizes, resulting in "low target loss." By capturing the beads in a lateral flow path, there is no cellular manipulation or washing away of nontarget cells. Sensitivity is as great as one in 1 billion cells, versus one in 10,000 cells for flow cytometry.
Throughout is also high1 mL (about 4 billion cells) can be processed in 30 seconds, compared to two or more hours for flow cytometry, he says. The recovery rate is as high as 90% of the target cells.
Therefore, Feistel says, "We can use readily available fluid-handling systems, and the technology can then become a high throughput cell sorter with sensitive detection devices for specimen volumes ranging from nanoliters to several milliliters. This is ideal for high content cellular assays."
X-ray chromatography research (XCR) kits to detect tumor cells in blood and bone marrow have just been released for drug discovery. XRC tool kits will be released for researchers later this year, allowing them greater flexibility.