Material and Methods
Cell seeding and transfection were performed using the Biomek FX automated workstation. For the transfection, X-tremeGENE HP and X-tremeGENE 9 reagents (Roche) were prediluted in Opti-MEM (dilution factor: 1:50 for X-tremeGENE HP reagent, 1:33 for X-tremeGENE 9 reagent). The EGFP-PC3.1 plasmid was prediluted in Opti-MEM (1 µg/100 µL). The transfection-complex was formed by pipetting the same amount of plasmid dilution into the dilution of transfection reagent, followed by mixing and incubation for 30 min.