Continuous Monitoring Provides Deeper Insight
Bionas 2500 can be used with many different cell types including cell lines, primary cell cultures, tissue slices, and biopsies to investigate drug candidates or to validate targets. Applications include elucidating the mechanism of action of a substance, validating targets, or conducting toxicity studies.
A better understanding of cellular physiology is possible through dynamic monitoring with this system than with any other technologies available today. Continuous measurements also allow the monitoring of distinct fluctuations in acidification rate, oxygen consumption, and cellular adhesion over an extended time period. In comparison, endpoint-based methods such as protein assays are inherently not capable of detecting these fluctuations.
The Bionas system also provides a physiological fingerprint of what happens inside the cells because of its unbiased set-up. Combining the results with those from other techniques “helps us to move forward,” says Eric Ronken, Ph.D., principal scientist at Solvay Pharmaceuticals (www.solvaypharmaceuticals.com). Solvay uses the Bionas system for target validation.
“Sometimes the most cited signal transduction pathways may not be the relevant ones. For example, cAMP is regulated by many receptors, yet sometimes we see that certain kinases are components of the true predictive signaling route of a specific neural circuit.”
With the Bionas 2500 system, Dr. Ronken observes a receptor-mediated uncoupling of acidification and oxygen consumption. “Using combinations of agonists and antagonists, in the absence or presence of certain inhibitors, we can get a nice fingerprint of signaling cascades. Furthermore, the resistance parameter responds in its own unique way, delivering yet another parameter that may be of relevance in distinct brain areas, the pituitary gland, or indeed in any other cell or tissue compartment that may respond to specific stimuli.”