Details of Cellular Responses
The system can be used to measure any stable fluorescent signal down to a resolution of 0.8 µm. However, certain organelles that cannot be resolved such as mitochondria, can be analyzed regardless. Analysis can be done using TMRE accumulation as an indicator of mitochondrial activation. Although individual mitochondria cannot be clearly resolved, the fluorescence around the nucleus of cells containing active mitochondria can be measured. This enables a rapid and quantitative measurement of active mitochondria.