1) Molecular Biology: As “Your Gene Company”, OriGene has a broad portfolio of biological reagents and services that are of value to both academic research and industry product development. Utilizing high-throughput technologies in molecular cloning, the human cDNA collection is nearing completion with respect to the NCBI RefSeq curated database of unique human transcripts. Such a comprehensive portfolio of cDNA clones is of particular value to laboratories that wish to move their projects along quickly by purchasing “ready-made” clones and where time is of the essence in mission critical research. If not immediately available, OriGene’s Blue Heron synthesis service can conveniently provide any gene, any sequence, in any vector, with add-on services of codon-optimization and large-scale DNA purification. The cDNA clone sets can be used for high-throughput functional screening to identify novel players in biological processes.
2) Protein Assay and Antibodies: OriGene’s 7,000 recombinant human proteins produced in human cells are of high value to researchers that are interested in authentic structure. Proteins with correct post-translational modifications (phosphorylation, glycosylation, etc.) are crucial for biologically relevant structure and functional studies. They are also ideal for assay development, small molecule interaction screening, or as antigens for antibody production.
Taking full advantage of the successful purification of these human proteins, OriGene has created manufacturing capacity to produce over 300 fully validated monoclonal antibodies per month (TrueMAB). This growing catalog of well over 5,000 antibodies is currently supplying leading researchers with highly specific antibodies directed against authentic human protein antigens with validation assurances for critical applications such as Western, flow, IP, IF, IHC, ELISA and for multiplexed assays.
3) Diagnostic Tests: To address the unresolved challenges of antibody cross-reactivity, OriGene has created a new generation of antibodies known as UltraMAB™ Antibodies. Each UltraMAB has been rigorously tested on OriGene’s proprietary high-density 10K protein lysate microarray to validate and confirm its mono-specificity against human protein antigens. (See image for HER2 UltraMAB data.)
This unmatched quality metric and novel approach to antibody validation is crucial for antibody-based applications, especially for diagnostic tests involving IHC and/or flow cytometry. The launch and expansion of the UltraMAB collection have been greatly appreciated by leading diagnostic partners and welcomed by many assay and companion-diagnostics developers challenged by the issues of cross-reactivity. This places OriGene in a unique position to serve as the preeminent partner and collaborator for diagnostic antibody development.