CHO cells have become one of the most widely used cell lines in bioprocessing today. With such a variety of clones in use, it is important to optimize each cell culture strategy to get the best performance from each clone.
One of the goals of this study was to increase product titer of selected CHO clones by extending the culture time as seen above.
Using various feed formulations and strategies, both fed-batch cultures were extended by 2 and 3 days resulting in an increased product titer over batch culture. Future studies will include the modification of process parameters by adjusting volume, temperature, agitation, and dissolved oxygen settings in bioreactor cultures. These process parameter optimization steps, along with base medium selection and feed strategy design, will result in each CHO clone attaining its peak performance in culture.
Process optimization along with a well-founded, fed-batch procedure can result in higher product titer levels in a variety of CHO clones. This begins with a versatile basal medium such as Thermo Scientific HyCell CHO, which is chemically defined, and supports high cell densities and increased productivity for a variety of CHO clones. When paired with chemically defined process feed supplements such as Thermo Scientific HyClone Cell Boosts, a considerable increase in product titer is possible.