Essen BioScience is a world leader in the field of cell-based in vitro assays and instrumentation. In its recent history, Essen has concentrated on imaging products with the release of the IncuCyte™ in 2006, and very recently with the release of the second-generation IncuCyte ZOOM™ in 2012. Along with a comprehensive line of CellPlayer™ kinetic assays and reagents and a renowned global Discovery Services team, Essen BioScience provides quantitative assay solutions in an array of research areas including oncology, neuroscience, and ion channels.
Traditional high-content imaging assays are conducted on live cells, however the assay readout is typically a single predetermined time point. With IncuCyte ZOOM, Essen offers a new type of imaging, Live Content Imaging, where the acquisition, analysis, and quantification of images are taken from living cells that remain unperturbed by the detection method, allowing for repeated measurements over long periods of time (days to weeks). This approach allows researchers to follow assays in real time, and further does not require the determination of an assay end point a priori.
An enabling aspect of the IncuCyte ZOOM technology is that the system acquires images automatically from within the user’s cell culture incubator, collecting biological image data around the clock on multiple vessels without removing the cells from their preferred physiological environment. The IncuCyte ZOOM is also equipped with integrated software providing real-time analysis of the biological processes under observation. The IncuCyte ZOOM features multifluorescent colors, support of a broad range of microscope objectives, and enhanced speed to handle the acquisition and processing demands of real-time image acquisition and analysis. The CellPlayer reagents include targeted fluorescent probes and fully validated GFP/RFP nuclear labeled stable cell lines. These reagents are nonperturbing to cell health and superior to traditional organic dye labels for long-term imaging assays. Together, the IncuCyte ZOOM and suite of CellPlayer assays and reagents provide a fully integrated solution for quantitative, kinetic biology.