Mass spectrometry (MS) coupled with liquid chromatography (LC) has emerged as the most versatile analytical strategy for targeted protein analysis. From monitoring large-scale production processes to the discovery and validation of protein biomarkers, the use of targeted MS for protein analysis is an appealing alternative to ELISA-based assays due to the ability to multiplex protein targets and achieve high levels of specificity and sensitivity. A key and often rate-limiting requirement for the implementation of MS-based protein analysis is sample preparation, which involves protein denaturation, reduction, alkylation, in-solution trypsinization, and peptide cleanup. Sample preparation is typically performed in a manual or semi-automated fashion that leads to variable results and is often the bottleneck in the entire protein analysis workflow.
In response, Agilent Technologies has developed solutions on the AssayMAP technology platform for sample preparation that facilitate reproducible, robust, high-throughput quantification by mass spectrometry or other high-performance detector. AssayMAP technology intelligently automates complex protein sample-preparation workflows, enabling larger campaigns, deployable methods, and improved data quality for methods that have been previously limiting.
The AssayMAP technology comprises three main components: a specialized Agilent Bravo Automated Liquid Handler equipped with positive displacement syringes, miniature chromatography cartridges packed with resin appropriate for each application, and an optimized protocol with a simple user interface that runs at the push of a button. Each workflow is designed to have maximum assay development flexibility controlled through simple inputs on a computer interface.