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January 01, 2014 (Vol. 34, No. 1)

Advances in Protein Expression

  • Approximately half of approved recombinant biotherapeutics are expressed in Escherichia coli . During downstream processing, it is necessary to remove the organism’s outer membrane, which consists of lipopolysaccharide (LPS), also known as endotoxin. LPS is involved in a diverse spectrum of immune-response pathologies. Standard methods for removing ...

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