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March 15, 2009 (Vol. 29, No. 6)

Advances in Label-Free Assay Screening

Advantages and Limitations of New Tools Such as RAP, Optical Biosensors, and SPR

  • Currently, most assays for detection of biologically relevant binding events use radioactive or fluorescent dyes to tag one or more molecules. The need for multiple reagents limits label-based assay flexibility and speed, particularly in HTS formats. In cell-based assays, label dependence may produce artifacts because of manipulation of ...

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