These studies demonstrate that the mosquito can accurately and reproducibly pipette nanoliter volumes from variable plate formats. Before integrating mosquito into the workflow, all pipetting for this project was carried out using multichannel pipettors or a 96-well pipetting instrument. Our research group subsequently used the Freedom to make serial dilutions (10 uL into 20 uL) and relied on mosquito to then transfer these ten-point dilutions into other plates for further use. This data demonstrated that mosquito is capable of carrying out both dilution steps with improved pipetting times of roughly two minutes per plate.
The provision of low-volume assay-ready plates and the improved accuracy of dose-inhibition curves have been accompanied by a significant decrease in unnecessary workload. Additionally, it reduced the volume of DMSO waste and decreased the amount of valuable compound used by up to 16-fold. mosquitos ability to miniaturize assays means much smaller volumes, resulting in saving on resources such as buffers, assay kits, radio-ligands, and enzymes. The use of this automated multichannel pipettor has also reduced the risk of human error by eliminating manual pipetting and the associated health and safety risks.
The mosquito enabled the screening of up to nine assay plates, or 72 compounds per run, and has supported a variety of assays, including viral, radio-ligand, enzyme, fluorometric imaging plate reader assays, and bioluminescent assays. In summary, mosquito is a cost-effective solution to optimize workflow and efficiency within the screening laboratory. Moreover, it has potential for additional applications in the drug discovery sector, including confirmation, specificity, cytotoxicity, and P450 assays. We have demonstrated that mosquito has the ability to make assay-ready plates and daughter plates for further analysis and we are now looking to integrate the use of mosquito into our crystallography projects.