3. Develop Effective Documentation
Generating effective documentation is another critical part of pre-transfer work. A detailed standard operating procedure (SOP) describing the assay as it is executed in the transferring site is key because it provides a standard for training and implementation at the receiving site.
Robust and detailed SOPs created at the transferring site provide information that can further define assay steps to reduce problems at the receiving site. Detailing the liquid-handling steps outlined in Table 1 will make it easier for the receiving site to mimic the transferring site and will ultimately make a successful transfer more likely.
Another important component is the transfer protocol, which is a formal protocol defining the expectations for the transfer exercise. It provides agreement between the sites on how the transfer will be executed and defines the criteria for a successful assay transfer. A comparison between the results for identical samples at both sites is required, and an equal number of determinations for each sample at each site are advisable to simplify statistical analysis.