GEN UPDATES in biotechnology:
Real-Time, Label-Free Cellular Analysis

GEN Technology Updates are composed of articles commissioned for publication by Genetic Engineering & Biotechnology News and of peer-reviewed scientific papers from journals published by Mary Ann Liebert, Inc. They are intended to provide in-depth information and coverage on cutting-edge technologies and trends that are transforming drug discovery and life science research.

Dynamic Label-Free Cell-Based Assays Using Real-Time Cell Electronic Sensing System
Josephine M. Atienza, Naichen Yu, Shelli L. Kirstein, Biao Xi, Xiaobo Wang, Xiao Xu, and Yama A. Abassi

Dynamic Detection of Natural Killer Cell-Mediated Cytotoxicity and Cell Adhesion by Electrical Impedance Measurements
Joakim Glamann and Anker Jon Hansen

Application of Real-Time Cell Electronic Sensing (RT-CES) Technology to Cell-Based Assays
Kelli Solly, Xiaobo Wang, Xiao Xu, Berta Strulovici, and Wei Zheng

TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN
Jon R. Inglefield, Calin Dan Dumitru, Sefik S. Alkan, Sheila J. Gibson, Kenneth E. Lipson, Mark A. Tomai, Chirs J. Larson, and John P. Vasilakos

Live Cell Quality Control and Utility of Real-Time Cell Electronic Sensing for Assay Development
Shelli L. Kirstein, Josephine M. Atienza, Biao Xi, Jenny Zhu, Naichen Yu, Xiaobo Wang, Xiao Xu, and Yama A. Abassi

Cell Proliferation
A functional screen identifies miR-34a as a candidate neuroblastoma tumor suppressor gene.
Cole KA, Attiyeh EF, Mosse YP, La Guaglia MJ, Diskin SJ, Brodeur GM, Mar JM. Mol Cancer Res 2008 May; 6(5): 735-42.

Identification of ALK as a major familial neuroblastoma predisposition gene.
Mossé, YP, Laudenslager M, Longo L, Cole KA, Wood A, Attiyeh EF, Laquaglia MJ, Sennett R, Lynch JE, Perri P, Laureys G, Speleman F, Kim C, Hou C, Hakonarson H, Torkamani A, Schork NJ, Brodeur GM, Tonini GP, Rappaport E, Devoto M, Maris JM. Nature advance online publication 2008, August 24; doi: 10.1038/nature07261.

Cytotoxicity and Cell Death
Microelectronic cell sensor assay for detection of cytotoxicity and prediction of acute toxicity.
Xing JZ, Zhu L, Gabos S, Xie L. Toxicol In Vitro. 2006 Sep; 20(6): 995-1004. Epub 2006 Feb 14.

A cell-microelectronic sensing technique for profiling cytotoxicity of chemicals.
Boyd JM, Huang L, Xie L, Moe B, Gabos S, Li X-F. Analytica Chimica Acta 2008 Apr; 615: 80–87.

Receptor Activity
Real-time monitoring of morphological changes in living cells by electronic cell sensor arrays: an approach to study G-protein coupled receptors.
Yu N, Atienza JM, Bernard J, Blanc S, Zhu J, Wang X, Xu X, Abassi YA. Anal Chem. 2006 Jan 1; 78(1): 35-43.

TLR7 agonist 852A inhibition of tumor cell proliferation is dependent on plasmacytoid dendritic cells and type I IFN.
Inglefi eld JR, Dumitru CD, Alkan SS, Gibson SJ, Lipson KE, Tomai MA, Larson CJ, Vasilakos JP. Journal of Interferon & Cytokine Research 2008; 28: 253–263.

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