BioPerspectives

Jun 28, 2007

Real Time Quantitative PCR (RT-qPCR) in a Clinical Reference Laboratory

  • Over the past decade, PCR testing for infectious agents in the clinical laboratory has begun to replace other methods, such as culture. PCR is often now referred to as the gold standard in this field. Further evolution of this technology has seen the introduction of Real Time Quantitative (RT-qPCR), replacing traditional gel detection methods for amplified products.

    Jeffery Stevenson, Ph.D., and his colleagues at the ARUP Institute for Clinical and Experimental Pathology are developing novel diagnostics for clinical and anatomic pathology in a wide range of disciplines. Increasingly, the group is relying on RT-qPCR technology. During this week's podcast, Dr. Stevenson discusses why RT-qPCR is such an attractive method for a clinical reference lab. He describes its major advantages and notes the potential pitfalls that need to be kept in mind when using the technique.

    Dr. Stevenson provides examples of how his lab uses RT-qPCR for detecting viral pathogens and how the data for these assays are validated. He lists the key points to consider when working on the development of an accurate and reproducible RT-qPCR assay and suggests improvements for making the technology even more valuable in a clinical reference lab.

    Listen to this highly informative podcast then return to the blog to give your comments on the following question:

    What improvements would you like to see in QPCR technology that might make it more valuable in a clinical reference laboratory?

    Or, if you prefer, post your own topic on any biotech industry subject you would like.