Gene Therapy: A New Twist on an Old Helix
Retooled and Resurgent, Gene Therapy Is Safely Delivering
RNA-Seq: Less Lumping, More Splitting
Single-Cell RNA-Seq Detects Subtle Differences between Cellular Subtypes, Demands Specialized Methods of Data Analysis
Literature Review: Virus Neatly Organizing Its Own Nucleus
Phage Infection and Replication in a Bacterial Host
Researchers Seek Ways to Minimize Off-Target Effects
Vectors (plasmids, cosmids, BACs, etc.) are certainly friends to the researcher—we all use these valuable DNA constructs to clone and manipulate our DNA sequences of interest. But, what happens when (dun, dun, DUN!) vectors turn against us?! OK, so it’s not quite that dramatic, but it is true that vector contamination is a legitimate threat when it comes to sequencing your precious DNA of interest. So how can you be sure that a particular DNA sequence—be it your own or one that you found online—is free from contamination? The NCBI actually offers an online resource for exactly that purpose. VecScreen allows users to input a query sequence (via Accession number, GI, or FASTA sequence) and search it against an extensive database of vector sequences. The search tool subsequently identifies fragments that may be of vector origin.