Leading the Way in Life Science Technologies

GEN Exclusives

More »

Best of the Web

More »
Jun 01, 2008 (Vol. 28, No. 11)


  • Useful primer info
  • Some design issues
Another approach to primer design for real-time quantitative PCR can be found at RTPrimerDB, where yet another database aims to provide reliable, validated primer sets for use in the method. It is not completely clear what the difference between this database and the previous one is, though RTPrimerDB appears to have more of an open-source feel to it. Researchers are encouraged to submit their own validated primers/probes for use in the database. Another visible difference is that browsing of primers/sequences is not enabled here. I found that rather annoying, as it required me to have gene ID information to identify primers. A rather simple search engine provides access to the information. Registration is required for full access to perform activities such as submitting sequences. While it doesn’t feel as extensive as the last database, it undoubtedly will have sequences the other does not, so the two will likely complement each other.
  • Key:
  • Strong Points
  • Weak Points
  • Ratings:
  • Excellent
  • Very Good
  • Good

*The opinions expressed are solely those of the author(s) and should not be construed as reflecting the viewpoints of the publisher, Genetic Engineering & Biotechnology News, Mary Ann Liebert, Inc., the publishing house, or employees and affiliates thereof.

Be sure to take the GEN Poll

Cancer vs. Zika: What Worries You Most?

While Zika continues to garner a lot of news coverage, a Mayo Clinic survey reveals that Americans believe the country’s most significant healthcare challenge is cancer. Compared to other diseases, does the possibility of developing cancer worry you the most?

More »