June 15, 2010 (Vol. 30, No. 12)

URL:
http://www.ludesi.com/free-tools/geliq

Rating:
Strong Points: Nice background information regarding the application
Weak Points: Nothing major

Summary:

Despite the fact that 3-D is a hot commodity at the moment—such as for films and television sets—one must remember that lowly 2-D technology serves a great purpose in its own right. Take gel electrophoresis for example. 2-D electrophoresis is a staple technique in proteomics, allowing the separation and characterization of proteins from a heterogeneous sample based on protein charge and molecular mass. The problem with the technology, though, is that fuzzy data spots on the gel can lead to fuzzy interpretation. To combat this, several research groups have developed the “combined correctness metric” to analyze 2-D gels. And, lucky for you, you don’t need to understand exactly how it works in order to take advantage of it, thanks to Ludesi’s free (that’s right—FREE!) Gel IQ application. This application works with your current 2-D gel analysis software (such as ImageMaster, PDQuest, REDFIN, and SameSpots) to determine how correct the analysis is. The Gel IQ website contains the download, as well as additional background information and tutorials.

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